Method 525.3

Method 525.3 was developed by the US EPA for the determination of semi-volatile organic chemicals in drinking water. This is an analytical method that uses solid phase extraction (SPE) and gas chromatography/mass spectrometry (GC/MS) for the identification and quantitation of 123 selected compounds. These compounds range from polychlorinated biphenyls (PCBs), poly aromatic hydrocarbons (PAHs), and pesticides. The standards can be purchased individually or as a complete set for your laboratory's particular needs. Additionally, the required surrogate and internal standards are also offered.

Method 525.3 Semi-Volatile Organic Chemicals in Drinking Water

PCB and PAH Standard

M-525.3-01 1 x 1 mL
500 µg/mL each in Acetone 30 comps.
2-Chlorobiphenyl Anthracene
4-Chlorobiphenyl Benz(a)anthracene
2,4'-Dichlorobiphenyl Benzo(b)fluoranthene
2,2',5-Trichlorobiphenyl Benzo(k)fluoranthene
2,4,4'-Trichlorobiphenyl Benzo(g,h,i)perylene
2,2',3,5'-Tetrachlorobiphenyl Benz(a)pyrene
2,2',5,5'-Tetrachlorobiphenyl Chrysene
2,3',4',5-Tetrachlorobipheny Dibenz(a,h)anthracene
2,3,3',4',6-Pentachlorobiphenyl Fluorene
2,3',4,4',5-Pentachlorobiphenyl Hexachlorobenzene
2,2',3,4,4',5'-Hexachlorobiphenyl Indeno(1,2,3-cd)pyrene
2,2',3,4',5',6-Hexachlorobiphenyl Isophorone
2,2',4,4',5,5'-Hexachlorobiphenyl Pentachlorophenol
2,2',3,4,4',5,5'-Heptachlorobiphenyl Phenanthrene
Acenaphthylene Pyrene

Phthalate Standard

M-525.3-05 1 x 1 mL
100 µg/mL each in Acetone 6 comps.
Benzyl butyl phthalate Diethyl phthalate
bis(2-Ethylhexyl)adipate Dimethyl phthalate
bis(2-Ethylhexyl)phthalate Dibutyl phthalate


M-525.3-06 1 x 1 mL
500 µg/mL in Acetone

Internal Standards

M-525.3-IS 1 mL
500 µg/mL in Acetone 3 comps.
Acenaphthene-d10 Phenanthrene-d10
M-525.3-IS2 1 x 1 mL
1000 µg/mL in MeOH

Surrogate Standard

M-525.3-SS 1 x 1 mL
500 µg/mL each in Acetone 3 comps.
1,3-Dimethyl-2-nitrobenzene Benzo(a)pyrene-d12

Pesticide Standards

M-525.3-02 1 x 1 mL
500 µg/mL each in Acetone 33 comps.
Gesatamine Chloroneb
Butachlor Dacthal
Butylate p,p'-DDD
Cycloate p,p'-DDE
Diphenamid p,p'-DDT
EPTC a-Chlordane
Metolachlor g-Chlordane
Molinate Methoxychlor
Tillam cis-Permethrin
Propachlor trans-Permethrin
Propazine Simazine
Simetryn trans-Nonachlor
Atrazine Chlorothalonil
a-BHC Diisopropyl methanephosphonate
b-BHC Profenofos
d-BHC Vinclozolin
M-525.3-03 1 x 1 mL
500 µg/mL each in Acetone 39 comps.
Ametryn Endosulfan sulfate
Chlorpropham Etridiazole
Chlorpyrifos Heptachlor
Dichlorvos Heptachlor epoxide (Isomer B)
Ethoprop 2,4-Dinitrotoluene
Fluridone 2,6-Dinitrotoluene
Hexazinone Hexachlorocyclopentadiene
Mevinphos Acetochlor
Norflurazon 2,6-Di-tert-butyl-p-cresol
Prometryne Chlorfenvinphos
Pronamide N,N-Diethyl-m-toluamide
Tebuthiuron Ethion
Terbutryne Parathion
Triadimefon Parathion-methyl
Trifluralin Nitrofen
Vernolate Phosphamidon
Alachlor Tebuconazol
Aldrin Tribufos
Chlorobenzilate Tetrachlorvinphos
M-525.3-04 1 x 1 mL
500 µg/mL each in Acetone 14 comps.
Bromacil Disulfoton
Cyanazine Endosulfan I
Fenarimol Endrin
MGK-264 Dimethipin
Napropamide Oxyfluorfen
Prometon Phorate
Terbacil Endosulfan II

Method 525.3 Semi-Volatile Set

M-525.3-SET 9 x 1 mL
M-525.3-01 M-525.3-06
M-525.3-02 M-525.3-IS
M-525.3-03 M-525.3-IS2
M-525.3-04 M-525.3-SS